ABSTRACT
Background: There has been marked rise in use of internet over the last decade, with increasing internet use people are more prone to psychological disorders. This has markedly hit the population of young adults as they are the ones who maximum use internet. These users are also prone to develop Attention deficit hyperactive disorder(ADHD) and other behavioural problems.Methods: The study was conducted by the Department of Psychiatry, 900 (design effect=2) students were randomly selected (620 from Medical College and 280 from Dental College). Out of 900, 618 students answered the questionnaire completely which included Young’s Internet Addiction Test (IAT) and the Adult ADHD Self-Report Scale (ASRS-v1.1). Subjects who came positive for ASRS were asked to undergo a test for DSM-V. Independent t-test was used to assess the outcomes and p<0.05 was considered significant.Results: The mean age of the participants were 20.03±1.45 years. It was found that people from age group 19-20 were addicted the most (240 students), coming next were from age group 21-22 (162 students). The prevalence rate of self-reported ADHD symptoms using the ASRS screener was 20.2%. Out of these ADHD suspects 39(31.45%) were moderately addicted and 73(58.87%) were mildly addicted, and this association was found to be statistically significant (p<0.001).Conclusions: Thus, with modernization it is seen that internet use has dramatically increased, which has various kinds of behavioural, psychological, mental effects on one's life. Moreover, students who were more addicted to internet were found to be more susceptible to ADHD.
ABSTRACT
BACKGROUND: Zanthoxylum heitzii is a spice used to prepare several dishes and to treat tumors, syphilis, malaria, cardiac palpitations, urogenital infections in the west region of Cameroon, but the antitumor mechanisms and chemical composition are not yet investigated. This study was aimed to determine the antiproliferative effects of four extracts from the fruits and barks of Zanthoxyllum heitzii (Rutaceae) on apoptosis in human promyelocytic cells, their mechanisms and the chemical composition. The 3-(4, 5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine the fifty percent inhibition (IC50) concentration of the cell lines after treatment. The effect on morphology was observed using a light or fluorescence microscopy. The rate of apoptosis and the cell cycle were measured using flow cytometry (FCM). The phytochemical analysis of the extract was carried with HPLC/MS methods. RESULTS: The phytochemical analysis of the extracts indicated the presence of four known polyphenols (Syringic acid, Juglon, Luteolin and Myricetin) in both fruits and barks of Z. heitzii but in different quantities. Syringic acid and Myricetin concentrations were between 17-21 fold higher in the fruits than the stem bark. Rhamnetin (393.35 µg/mL) and Oleuropein (63.10 µg/mL) were identified only in the stem barks of Z. heitzii. Among the four extracts tested for cytotoxicity properties, only the methanol extract of fruits and barks significantly inhibited cell proliferation of HL-60 cells with IC50 value of 20 µg/mL and 12 µg/mL respectively. HL-60 cells treated with Z. heitzii extracts significantly produced reactive oxygen species (ROS) with concurrent loss of mitochondrial membrane potential (MMP). Modifications in the DNA distribution and enhanced of G1/G0 phase cell cycle arrest were observed in a concentration dependent manner. CONCLUSIONS: Polyphenols from Z. heitzii plant exert inhibitory effect on HL-60 cells through the reactive oxygen species (ROS) generation, loss of mitochondrial membrane potential and cell cycle destabilization.